Cores for Vision Research Administration Ocular Microanatomy Core (OM Core) Visual Function and Non-invasive Ocular Imaging Core (VFNOI Core) Ocular Mass Spectrometry, Lipidomics, and Proteomics Core (OMSLP Core) Ophthalmic Testing Core (OTC) Visual Function and Non-invasive Ocular Imaging Core (VFNOI Core) Home Research Cores For Vision Research Visual Function and Non-invasive Ocular Imaging Core (VFNOI Core) Expansion of Vision Research Cores of Vision Research within the University of California Irvine (UCI) will strengthen and enhance NEI R01-funded research. The project aims to support the major expansion of vision research currently underway at UCI. Our goal is to broaden and optimize research capabilities, support and expand existing research projects, and help establish new studies, ultimately boosting productivity and strengthening vision research at UCI. It is sponsored by the NEI P30 grant, P30EY034070-01. Initial Service Request Form Vladimir Kefalov, PhD Professor, Gavin Herbert Eye Institute Ophthalmology Xiuli Ma Core Manager, Visual Function & Non-invasive Ocular Imaging Core Reservation sign-up for Equipment use Most equipment needed for testing living animals in the Visual Function and Non-Invasive Ocular Imaging Core is located in the animal housing facility where investigators’ animals are housed (Biological Sciences III; 519 Ring Rd). The room holding this equipment is immediately adjacent to our animal rooms, and is located within a 5-minute walk from the laboratories of all CTVR investigators. The housing of this equipment within the animal facility makes testing the visual function of living animals convenient and efficient, particularly in longitudinal studies involving multiple data points before and after drug or genetic treatments. The rest of the VFNOI Core equipment, used solely for imaging of the anterior eye, is located in the Hewitt Building, where it is conveniently adjacent to the laboratories of investigators who study the anterior eye. Because of its specialized nature and requirements for a controlled environment, the 2-photon microscope is housed in a dedicated space on the 2nd floor of the Hewett Building. Reservation sign-up for equipment use Major Equipment Equipment for visual function and non-invasive ocular imaging for posterior eye studies is located in our animal facility Biological Sciences III; and equipment for anterior eye studies in located in Hewitt Hall. Both locations are convenient and place the equipment in the vicinity of the animals or of the laboratories involved in that research, respectively. Biological Sciences III Diagnosys Celeris is a rodent in vivo electroretinography (ERG) system that combines an electrode and stimulator into one device. This unit requires minimal training for use and enables the rapid collection of reliable and reproducible ERG results from rodents. UTAS BIGSHOT Visual Diagnostic System from LKC Technologies is another rodent in vivo ERG system that provides highly uniform ganzfeld stimulation and can perform flash ERG recordings in scotopic, mesopic, and photopic conditions. Bioptigen Envisu C-Class is a spectral domain optical coherence tomography (SD-OCT) system that provides high-resolution, real-time images with detail down to 3 μm and featuring full 3D volumetric data capturing capability with fully customizable high density data scans up to 1000 x 1000 of animal ocular tissue. Bioptigen Envisu R-Series, R4110 is another SD-OCT system that provides full-volume detail and visualization with high signal-to-noise-ratio for animal ocular tissue studies. Scanning Laser ophhalmoscope (SLO): Heidelberg Engineering, HRA2 (laser class 1) is an expandable diagnostic imaging platform which combines scanning laser fundus imaging with high-resolution OCT. It provides high image contrast while requiring low light exposure for fluorescein and ICG angiography, as well as reflectance autofluorescence and 3D scanning. PhenoSys qOMR is an automated quantitative Optomotor Response System that allows rapid and automated screening of functional vision using the optokinetic tracking response. Because of its fully automated nature, the system requires minimal training and enables the rapid collection of results and repetitive screening of animals in longitudinal studies. Hewitt Hall Heidelberg Instruments HRT II with the Rostock Corneal Module is a confocal scanning microscope that provides high-resolution images of the cornea and external ocular structures. This is built on their Retinal Tomography platform and can also be used to image the mouse retina. Tandem Scanning Confocal microscope (Tandem Scanning Corporation, Reston, Virginia. Model 165A) is a reflection-based confocal microscope that is used to volumetrically image the cornea and ocular surface cells. Bausch & Lomb’s Orbscan IIz is a corneal topography system that evaluates the anterior and posterior corneal surface topography as well as thickness from limbus to limbus. Our 2-photon microscope is a customized Leica TCS SP8 for in vivo and ex vivo imaging of eye structures and tissues. The device is equipped with 3 HYD detectors, 2 PMT detectors a Vitara T 20 femtosecond laser and a Vision S 75 femtosecond laser to enable fluorescence intensity and lifetime-based imaging of endogenous and exogenous fluorophores. We have 2 optical coherence tomography (OCT) units (a Bioptigen SDOIS OCT, and a Bioptigen Envisu unit). Heidelberg HRA2 Optical coherence tomography/scanning laser microscopy PhenoSys qOMR unit to measure retinal function Slide 1 Slide 2 Slide 3